Washingtoncontrolled clinical comparison of isolator and bactec 9240. The systems evaluated were the bactec 9240 bactec and bactalert 3d bact. This system has been shown to be more sensitive than other systems for the detection of a. The occurrence of falsepositive blood cultures in patients with acute myeloid leukemia has been rarely described in the literature. Rapid identification and antimicrobial susceptibility testing. Prospective study of the clinical performance of three. A reading was considered to be false positive when it was not. Bd bactec instrumented blood culture systems from bd. The culture bottles used were the aer and the anaer culture bottles 8, both of which were incubated for 5 days. Indian j med microbiol serial online 2016 cited 2020 apr 17.
Superior detection of pathogens in synovial fluid by the bactec 9240 peds plusf system compared to the conventional agarbased culture method. Cryptococcal capsule antigen was performed on plasma. In a study from belgium of 1,743 persons with fever acquired in the tropics, only falciparum malaria resulted in deaths 2. Pdf comparison of bactec 9240 peds plus medium and. Direct comparison of the bactec 9240 and bactalert 3d automated blood culture systems for candida growth detection. The binaxnow staphylococcus aureus testing showed sensitivity, specificity, and positive and negative predicative values of 97. Bactec 9240 blood culture system becton dickinson diagnostic instrument systems, sparks, md. Time to positivity of microorganisms with bactec 9050. The aerobic, anaerobic, and mycology media for each system were evaluated.
Analysis of the rate and amount of co 2 increase enables the bd bactec fluorescent series instrument to determine if the vial is positive, i. The present study demonstrates that 100% of 4h urease tests on signalpositive blood culture broths bactec 9240 containing gramnegative coccobacilli or no visible organisms were. Internationaljournalof immunopathologyand pharmacology vol. Blood cultures are essential in the diagnosis and treatment of the etiologicagents of sepsis.
Pdf rapid diagnosis of brucella bacteremia by using the. The bactec 9240 was compared with the bactec nr 660 for the detection of organisms and bacteremic episodes. Patrick cy woo, the university of hong kong, hong kong. A total of 28 clinical isolates of escherichia coli, staphylococcus aureus, candida albicans, and candida glabrata were studied. Awareness of this finding is important to avoid unnecessary delays in initiating appropriate cytoreductive therapy. Blood volume in bactecplusf culture bottles sampled using the. Comparison of bactec 9240 peds plus medium and isolator 1. Multicenter comparison of bactec 9050 and bactec 9240 blood culture systems article pdf available in journal of clinical microbiology 366.
Prospective study of the clinical performance of three bactec media in a modern emergency department. Fluorescent technology in the bd bactec automated blood. The overall recovery of organisms and time to detection with the bactec 9050 and bactec 9240 systems were compared in a multicenter evaluation. Of 1,609 likely skin contaminants, 42 were recovered on day 5, 34 on day 6, 16 on day 7, and 5 on day 8. Bd bactec instrumented blood culture systems from bd diagnostics description bd diagnostics, the leader in blood culture instrumentation for the last 25 years, brings you the. The sensitivity and time to detection of brucella melitensis by the bactec 9240 and the isolator blood culture systems were compared in a prospective volumecontrolled study. Use of bactec 9240 blood culture system for detection of. From bactec 9240 blood cultures, mixed aliquots of blood and broth containing grampositive cocci in clusters were centrifuged at low speed to sediment red blood cells. Evaluation of bactec 9240 blood culture system by using. Extra vial sequence barcode labels can be used to replace.
Therefore, a three hospital retrospective study was conducted to evaluate overall positivity rates, isolation of specific pathogen groups, and contamination rates as a result of converting to vt from the bd system. Portions of the specimens were treated with an antibiotic or antifungal drug at specified concentrations to simulate antibacterial drug. Newsletter best practices in blood culture collection. Blood cultures are essential in the diagnosis and treatment of the etiologic agents of sepsis.
Bd bactec 9120 9240 instruments can also be easily combined with bd bactec fx modules via bd epicenter tm. Principlethe detection of microorganisms in a patients blood has diagnostic and prognosticimportance. Bactec mycof lytic medium a new medium which can be used with the bactec 9240 91209050 blood culture systems or the bactec 9000mb mycobacteria culture system. Times to detection of bacteria and yeasts in bactec 9240. Bd bactec 9120 9240 instruments can also be easily combined with bd bactec fx modules via bd epicenter.
Principle the detection of microorganisms in a patients blood has diagnostic and prognostic importance. Bactec plus blood culture bottles were preincubated at 35c or at room temperature before entry into the bactec 9240 instrument to determine the influence of preincubation temperature and time. Reference with high crp and leucocytosis especially in hospitalized source not found. B bactec plus aerobicf culture vials soybeancasein. There was no significant difference in the recovery of individual groups of organisms with the two systems, with the exception of. Each organism was tested in triplicate at 0, 8, 24, 36, and 48 h and was incubated at both room temperature rt and 35 degrees c. The combination of the bd bactec plus aerobic and lytic anaerobic media allows for improved time to detect and recovery of both aerobic and anaerobic organisms.
Becton dickinson bactec 9240 microbiological culture analyzer. Culture with bactec peds plusf bottle compared with. The bd bactec fx builds on the proven superior fluorescence detection technology, exceptional media performance and instrument reliability of the bd bactec 9000 blood. The bactec 9240 provides continuous testing of blood cultures through noninvasive fluorescent technology. Post a free request for proposal for a becton dickinson bactec 9240 445475 blood culture system and get quotes from our gold service dealers. Samples of simulated bacteremia were prepared using 10 ml of fresh blood from healthy humans and bacterial suspensions of known cell density. Severe malaria and artesunate treatment, norway to the editor. In the first phase of the study, a total of 4,383 compliant aerobic plus aerobicf blood culture sets were processed. Inoculation size was based on quantitative aspects of septicaemia as described previously yagupsky 1990.
A world of difference in blood culture page 3 072014 figure 2 fluorescent technology and sophisticated. The bottles were inoculated with five ml hhorse blood and incubated in the respective blood culturebc system bactalertalert 3d biomerieux and bactec 9240 bd until signalling for positivity or for a maximum of five days. Bactec 9240 and difco esp aerobic and anaerobic vials were inoculated with human blood and were seeded with organism growth from 18 species, including obligate. The bactec 9240 blood culture system with a standard aerobic medium, plus aerobicf, was compared to the bactalert system with a plateletspecific medium, bpa, as a means for. The bd bactec fx builds on the proven superior fluorescence detection technology, exceptional media performance and instrument reliability of the bd bactec 9000 blood culture systems. The most compact, innovative system design for maximum ergonomics and laboratory space.
Evaluation of bactec 9240 blood culture system by using high. If the subculture grows, edit the bottles status to positive on the edit test results screen. Comparison of bactec 9240 and difco esp blood culture systems for detection of organisms from vials whose entry was delayed article pdf available in journal of clinical microbiology 343. The performance of the four anaerobic blood culture bottles bact.
Bd bactec tm 9000 series bd bactec tm instruments product size description code 445475 1 bd bactec tm blood culture system bd bactec tm 9240 features include continuous monitoring of culture specimens, noninvasive design, unique fluorescent sensor technology, reliability and robustness, and simple operation. Health care solutions through microbiology introducing. A dedicated fungal culture medium is useful in the diagnosis. Principlethe detection of microorganisms in a patients blood has diagnostic and. Decrease time to detection during the analytical phase with a full spectrum of blood culture media solutions. The bactec 9240 was compared with the bactec nr 660. Use of bactec 9240 blood culture system for detection. Use of the bactec 9240 system with mycosisicf blood culture bottles for detection of fungemia. The purpose of this investigation was to evaluate the performance of the bactec 9240 and bactalert 3d blood culture systems in the detection of candida spp. In this study, we evaluated the performance of three blood culture systems in a chinese tertiarycare hospital.
Detection of simulated candidemia by the bactec 9240 system with plus aerobicf and anaerobicf blood culture bottles article pdf available in journal of clinical microbiology. Comparison of bactec 9240 and difco esp blood culture. After additional centrifugation and wash steps, pcr was performed on the resuspended pellet. Multicenter comparison of bactec 9050 and bactec 9240 blood. The most efficient, intuitive workflow for reduced handson time. Bacterial sepsis constitutes one of the most serious infectious. Approximately 15 to 20 cc of blood are drawn from the patient and injected into two bactec vials 9 to 12 cc in an aerobic bottle with resin, 3 to 6 cc in an anaerobic bottle. The bactec 9050 series of blood culture instruments are designed for the rapid detection of. Direct comparison of the bactec 9240 and bactalert 3d. Analysis of samples processed in automated blood culture system with blood culture samples processed by conventional manual method. The performance of the four anaerobic blood culture bottles. To evaluate the versatrek trek diagnostic systems, cleveland, ohio blood culture system against the bactec9240 bd microbiology, cockeysville, md, for the recovery of bloodstream pathogens. Plus aerobicf, plus anaerobicf, and anaerobic lyticf. Failure of an automated blood culture system to detect nonfermentative gramnegative bacteria.
Pdf comparison of bactec 9240 and difco esp blood culture. In conclusion, bactec lytic bottles have significantly better detection rates and shorter ttd. Synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic peds plus blood culture bottle and monitored by the bactec 9240 instrument for 4 weeks. Comparison of bactec 9240 and difco esp blood culture systems for the detection of organisms from vials whose entry was delayed. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page.
Conflicts that the editors consider relevant to the content of the manuscript have been disclosed. Early detection of bacteremia in the neonatal intensive. An evaluation was undertaken to determine the utility of the bactec peds plusf bottle and the bactec 9240 instrument becton dickinson diagnostic instrument systems. The bottles were read by the bactec 9240 fluorescence analyzer over a 7day incubation period. Block scientific is an acclaimed distributor in the united states, offering a fine line of lab equipment for sale including chemistry analyzers, hematology analyzers, centrifuges, histology equipment, reagents, consumables, and more used bactec 9240 automated blood culture system. Used bactec 9240 microbiological system block scientific. The performance of the four anaerobic blood culture. Frontiers comparison of the performance of three blood. Pdf direct comparison of the bactec 9240 and bactalert. Table table1 1 illustrates the comparison between the pcr results and those obtained using bactec 9240 and reveals a high level of agreement between the two methodologies, with.
Pdf superior detection of pathogens in synovial fluid by. Positive blood cultures by amplification and sequencing of. Here, we present the case of a 70yearold male with acute leukemia and persistently positive blood cultures despite broadspectrum antibiotic. In conclusion, selection of the bactec 9240 together with the pluslytic media combination was associated with increased positivity rates, significantly more septicemia drg codings and a. Bactec plus aerobicf, plus anaerobicf, and mycof lytic bottles, respectively, and bact fa, sn, and mb bottles. The dedicated fungal culture bottle was the myco culture bottle 9, which was incubated for 14 days in accordance with our laboratorys protocol.
Used becton dickinson bactec 9240 bd bactec 9240 microbiology. This blood culture analyzer is in very good conditions and sold as is. Analysis of samples processed in automated blood culture. Bd is empowering healthcare professionalsfrom specimen collection to actionable resultsto address the health and financial burdens associated with sepsis. A total of 1,072 synovial fluid cultures were processed, and 15 0. Comparison of 16s rrna gene pcr and bactec 9240 for. With the newer techniques of culture analysis such as the bactec 9240 fluorometric detection system, detecting bacteremia in the neonate may be possible in a. Bd is empowering healthcare professionalsfrom specimen collection to actionable resultsto address the. Comparison of the versatrek blood culture system against the. Venous blood from patients with suspected bacterial sepsis was evenly distributed into bottles of each system. Pdf detection of simulated candidemia by the bactec 9240. Rapid diagnosis of brucella bacteremia by using the bactec 9240 system article pdf available in journal of clinical microbiology 3510. A 7day incubation protocol was instituted with the bactec 9240 system for a 1year period to determine the times to detection of clinically relevant organisms. A world of difference in blood culture page 3 072014 figure 2 fluorescent technology and sophisticated algorithms.
Of 463 positive blood culture sets, 956 bottles were positive, of which the instrument detected 92. Positive signals were recorded and bottles processed onto standard media for organism. Comparison of 2 blood culture media shows significant. Rapid identification of staphylococcus aureus directly from.
Blood culture media thermo scientific signal blood culture system detect the growth of aerobic, anaerobic and microaeophilic organisms from blood samples with this unique one. Manufacturer specifications bactec 9240, tomy please note. This testing was repeated in each bactec fluorescentseries blood culture instrument bactec fx, 9240, 9050. Bactec 9240 system bd becton dickenson, sparks, maryland, usa. William johnston and colleagues from purdue university founded a company that specialized in developing instruments to detect very low levels of carbon14, which were marketed for use in air quality testing. The bd bactec vials are ready for use as received and require no reconstitution or dilution.
Each organism was inoculated at 10 to 100 cfu per bottle, across two blood volumes 3 ml and 10 ml over three lots of media for paired sets of the new and predicate devices. Approximately 8,000 cases of imported falciparum malaria are reported in europe each year 1. Comparison of the two blood culture systems, bactec 9240 and. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. The automated blood culture system used was the bactec 9240 series. After a 15min incubation, the bacteria were harvested and transferred to the appropriate panel of a bd phoenix automated microbiology system becton dickinson for identi.
Synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic peds plus blood culture bottle and monitored by the bactec. This study validates the use of the bactec 9240 continuously. Pdf multicenter comparison of bactec 9050 and bactec 9240. Used becton dickinson bactec 9240 445475 blood culture system. Manufacturer specifications bactec 9240, becton dickinson please note. Critical factors in the recovery of pathogenic microorganisms. The bactec 9240 blood culture system becton dickinson diagnostic instrument systems, sparks, md. Experience accumulated with the bactec 9240 instrument has demonstrated that the system enables detection of b. Block scientific is an acclaimed distributor in the united states, offering a fine line of lab equipment for sale. The bactec 9240 blood culture system uses infrared spectrophotometry to monitor carbon dioxide produced by microorganisms on a continuous basis. Impacting lives with a full spectrum of blood culture solutions. A direct comparison of two automated blood culture systems was conducted to compare their ability to detect candida growth.
1583 1508 960 829 943 1491 1050 1047 658 1594 1136 1201 487 763 1096 795 178 1446 1559 1620 436 401 887 216 596 1001 766 356 1128 1133 29